PhD-student in Cellular and synaptic phenotyping of human neurons from children with ASD

Vrije Universiteit Amsterdam
  • PhD-student in Cellular and synaptic phenotyping of human neurons from children with ASD
  • University Graduate
The CNCR in Amsterdam (see www.cncr.nl) participates in the Graduate School ONWAR (www.ONWAR.nl) and is seeking applications for a

PhD-student in cellular neuroscience
(cell biology, neurophysiology)
Department of Functional Genomics, VU/VUmc Amsterdam


Location: AMSTERDAM
FTE: 1 - 1

Job description

ASD is a developmental disorder for which currently no general effective therapy exists. This project is part of a larger program “New, rational therapy decisions for developmental brain disorders: Integrating quantitative patient assessment, cellular assays and nanotechnology (NewTDec)”, and is among the first project to operate under the Dutch research agenda (www.wetenschapsagenda.nl). NewTDec is a multidisciplinary consortium in which clinicians, neuroscientists, nanoscientists and patient organizations collaborate to develop new methods to identify biological causes for autism with the aim to develop personalized medicine for children with ASD. Quantitative assessment of brain functions using predictive EEG ‘signatures’ for individual patients and cellular assays using patient-own, stem cell-derived neurons will be used to measure specific brain characteristics in the same patient. This information is used in clinical trials to match patients with an optimal, individual therapy and to develop novel therapies for specific patient subgroups in the future.   

In this project we will use patch-clamp electrophysiology to study synaptic transmission and neuronal function in networks from inducible pluripotent stem cells (IPSC's) derived neurons from children with ASD. We will specifically investigate cellular and synaptic parameters that affect the balance between excitation and inhibition (E/I) in these networks. One of the leading hypothesis is that E/I balance is disturbed in neurodevelopmental disorders such as ASD, leading to altered information processing in the brains of these patients. We will test if and how cellular and synaptic parameters are changed in patients that have been selected for a putative altered E/I balance, based on biomarkers obtained from their EEG profile. Furthermore, we will test how these parameters are affected by various E/I modifying drugs. This information will eventually be used to inform N=1 clinical trials in the same patients. You will be trained on site and in specialized courses on campus. During the PhD you will write several scientific publications that will be combined in a PhD thesis.

Requirements

We are looking for candidates that hold, or will soon hold, a master degree in (Medical) Biology, Biophysics or Physics, preferably with hands-on experience in the methods listed above, and a strong motivation to pursue a career in science. Experience with Matlab or Python is an advantage.

What are we offering?

A challenging position in a socially involved organization. The salary will be in accordance with university regulations for academic personnel and amounts €2,395 (PhD) per month during the first year and increases to €3,061 (PhD) per month during the fourth year, based on a full-time employment. The job profile: is based on the university job ranking system and is vacant for at least 1 FTE.

The appointment will initially be for 1 year. After a satisfactory evaluation of the initial appointment, the contract will be extended for a total duration of 4 years.
Additionally, Vrije Universiteit Amsterdam offers excellent fringe benefits and various schemes and regulations to promote a good work/life balance, such as:
  • a maximum of 41 days of annual leave based on full-time employment
  • 8% holiday allowance and 8.3% end-of-year bonus

About Vrije Universiteit Amsterdam

The ambition of Vrije Universiteit Amsterdam is clear: to contribute to a better world through outstanding education and ground-breaking research. We strive to be a university where personal development and commitment to society play a leading role. A university where people from different disciplines and backgrounds collaborate to achieve innovations and to generate new knowledge. Our teaching and research encompass the entire spectrum of academic endeavour – from the humanities, the social sciences and the natural sciences through to the life sciences and the medical sciences.

Vrije Universiteit Amsterdam is home to more than 26,000 students. We employ over 4,600 individuals. The VU campus is easily accessible and located in the heart of Amsterdam’s Zuidas district, a truly inspiring environment for teaching and research.

Diversity
We are an inclusive university community. Diversity is one of our most important values. We believe that engaging in international activities and welcoming students and staff from a wide variety of backgrounds enhances the quality of our education and research. We are always looking for people who can enrich our world with their own unique perspectives and experiences.

The Faculty of Science
The Faculty of Science inspires researchers and students to find sustainable solutions for complex societal issues. From forest fires to big data, from obesity to medicines and from molecules to the moon: our teaching and research programmes cover the full spectrum of the natural sciences. We share knowledge and experience with leading research institutes and industries, both here in the Netherlands and abroad.

Working at the Faculty of Science means working with students, PhD candidates and researchers, all with a clear focus on their field and a broad view of the world. We employ more than 1,250 staff members, and we are home to around 6,000 students.

About the project
Project: Cellular and synaptic phenotyping of human neurons from children with ASD.
The project is part of the ZonMW program Dutch Science Agenda (NWA).

Description of work
The project exploits induced pluripotent stem cell (iPSC) technology to produce human neurons and create human neuronal networks in vitro. Genome editing using the CRISPR-Cas system is used to introduce disease-relevant variations in control cell lines or repair monogenic disease mutations in patient cells. Patch-clamp physiology is used to study the electrophysiological properties of the iPSC derived neurons. All the methodology, cell models, equipment, analysis software, viral vectors and technical support are available from the start of the project. 
 

Application

Are you interested in this position? Please apply via the application button and upload your curriculum vitae and cover letter until November 20, 2020.

Applications received by e-mail will not be processed.

Vacancy questions
If you have any questions regarding this vacancy, you may contact:

Name: L.N. Cornelisse
Position: Supervisor project
E-mail: l.n.cornelisse@vu.nl


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